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The 100 most highly cited papers of all time

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To mark the anniversary, Nature magazine asked Thomson Reuters to list the 100 most highly cited papers of all time. The search covered all of Thomson Reuter’s Web of Science, an online version of the SCI that also includes databases covering the social sciences, arts and humanities, conference proceedings and some books. It lists papers published from 1900 to the present day.

 
Title of the paperRankSujet
Phosphorus assay in column chromatography.81Biology lab techniqueVoir plus
MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures.82CrystallographyVoir plus
Van der Waals volumes and radii.83CrystallographyVoir plus
A new and rapid colorimetric determination of acetylcholinesterase activity.84Biology lab techniqueVoir plus
Projector augmented-wave method.85Physical chemistryVoir plus
Optimization by simulated annealing.86Physical chemistryVoir plus
Nitric oxide: physiology - pathophysiology - and pharmacology.87Biology lab techniqueVoir plus
An algorithm for least-squares estimation of nonlinear parameters.88Mathematics/statisticsVoir plus
Efficiency of ab initio total energy calculations for metals and semiconductors using a plane-wave basis set.89Physical chemistryVoir plus
A low-cost - high-efficiency solar-cell based on dye-sensitized colloidal TiO2 films.90Physical chemistryVoir plus
A low-viscosity epoxy resin embedding medium for electron microscopy.91Biology lab techniqueVoir plus
The Protein Data Bank.92CrystallographyVoir plus
Accurate and simple analytic representation of the electron-gas correlation-energy93Physical chemistryVoir plus
Rapid alkaline extraction procedure for screening recombinant plasmid DNA.94Biology lab techniqueVoir plus
Improved methods for building protein models in electron-density maps and the location of errors in these models.95CrystallographyVoir plus
Accurate spin-dependent electron liquid correlation energies for local spin-density calculations — a critical analysis.96Physical chemistryVoir plus
Continuous cultures of fused cells secreting antibody of predefined specificity.97Biology lab techniqueVoir plus
Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man.98MedicineVoir plus
Adsorption of gases in multimolecular layers.99PhysicsVoir plus
MrBayes 3: Bayesian phylogenetic inference under mixed models100PhylogeneticsVoir plus
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