The 100 most highly cited papers of all time

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To mark the anniversary, Nature magazine asked Thomson Reuters to list the 100 most highly cited papers of all time. The search covered all of Thomson Reuter’s Web of Science, an online version of the SCI that also includes databases covering the social sciences, arts and humanities, conference proceedings and some books. It lists papers published from 1900 to the present day.

 
Title of the paperRankSujet
Confidence limits on phylogenies: an approach using the bootstrap41PhylogeneticsVoir plus
A new generation of Ca2+ indicators with greatly improved fluorescence properties42Biology lab techniqueVoir plus
Efficient iterative schemes for ab initio total-energy calculations using a plane-wave basis set.43Physical chemistryVoir plus
High-resolution 2-dimensional electrophoresis of proteins.44Biology lab techniqueVoir plus
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.45PhylogeneticsVoir plus
Fuzzy sets.46Mathematics/statisticsVoir plus
Phase annealing in SHELX-90: direct methods for larger structures.47CrystallographyVoir plus
Clinical diagnosis of Alzheimer’s disease: Report of the NINCDS-ADRDA Work Group under the auspices of Department of Health and Human Services Task Force on Alzheimer’s Disease.48MedicineVoir plus
Special points for Brillouin-zone integrations.49PhysicsVoir plus
Study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.50Biology lab techniqueVoir plus
The CES-D scale: a self-report depression scale for research in the general population.51Psychology/psychiatryVoir plus
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.52Biology lab techniqueVoir plus
A rating scale for depression.53Psychology/psychiatryVoir plus
An inventory for measuring depression.54Psychology/psychiatryVoir plus
A simple method for displaying the hydropathic character of a protein.55Biology lab techniqueVoir plus
Determination of serum proteins by means of the biuret reaction.56Biology lab techniqueVoir plus
Maximum likelihood from incomplete data via EM algorithm.57Mathematics/statisticsVoir plus
Equation of state calculations by fast computing machines.58Mathematics/statisticsVoir plus
Controlling the false discovery rate: a practical and powerful approach to multiple testing.59Mathematics/statisticsVoir plus
Measurement of protein using bicinchoninic acid.60Biology lab techniqueVoir plus