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The 100 most highly cited papers of all time

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To mark the anniversary, Nature magazine asked Thomson Reuters to list the 100 most highly cited papers of all time. The search covered all of Thomson Reuter’s Web of Science, an online version of the SCI that also includes databases covering the social sciences, arts and humanities, conference proceedings and some books. It lists papers published from 1900 to the present day.

 
Title of the paperRankSujet
Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method.21Biology lab techniqueVoir plus
Revised effective ionic radii and systematic studies of interatomic distances in halides and chalcogenides.22Physical chemistryVoir plus
Processing of X-ray diffraction data collected in oscillation mode.23CrystallographyVoir plus
Regression models and life-tables.24Medical statisticsVoir plus
Density-functional exchange-energy approximation with correct asymptotic-behavior.25Physical chemistryVoir plus
Colorimetric method for determination of sugars and related substances.26Biology lab techniqueVoir plus
Use of lead citrate at high pH as an electron-opaque stain in electron microscopy.27Biology lab techniqueVoir plus
The CLUSTAL_X Windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools.28BioinformaticsVoir plus
Statistical methods for assessing agreement between two methods of clinical measurement.29Medical statisticsVoir plus
Reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.30Biology lab techniqueVoir plus
Isolation of biologically-active ribonucleic-acid from sources enriched in ribonuclease.31Biology lab techniqueVoir plus
The attractions of proteins for small molecules and ions.32Biology lab techniqueVoir plus
The moderator–mediator variable distinction in social psychological-research — conceptual - strategic - and statistical considerations.33Psychology/psychiatryVoir plus
Self-consistent equations including exchange and correlation effects.34Physical chemistryVoir plus
Rapid colorimetric assay for cellular growth and survival — application to proliferation and cyto-toxicity assays.35Biology lab techniqueVoir plus
Helical microtubules of graphitic carbon.36PhysicsVoir plus
The colorimetric determination of phosphorus.37Biology lab techniqueVoir plus
Disc electrophoresis — II. Method and application to human serum proteins.38Biology lab techniqueVoir plus
Inhomogeneous electron gas.39Physical chemistryVoir plus
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.40Biology lab techniqueVoir plus
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